# The evidence behind the Manapol® powder standard

> Who tested Manapol® powder, how it was tested, and what the Complex Carbohydrate Research Center reported.

- Page: https://aloe.mannatech.com/manapol/science/
- Landing: https://aloe.mannatech.com/manapol/
- Publisher: Mannatech, Incorporated
- Laboratory: Complex Carbohydrate Research Center (CCRC), University of Georgia
- Report date: 12/06/2022
- CCRC code: TS111122D
- Sample: Manapol CRMANE291102
- Project title: Glycosyl composition analysis and determination of the molecular weight of Manapol CRMANE291102
- Principal investigator: Tait Switzer, Mannatech, Incorporated
- Report prepared by: Artur Muszynski, PhD, MSc
- Report reviewed by: Christian Heiss
- Funding: Analysis commissioned and paid for by Mannatech, Incorporated; CCRC work also supported by U.S. DOE award #DE-SC0015662

## Methods

1. Molecular weight by Size Exclusion Chromatography (SEC) — sorts the sample by molecular size. This is what shows how much of the powder sits in the 1–2 million Dalton range that defines acemannan.
2. Glycosyl analysis (sugar profile) by GC-MS — breaks polysaccharides into individual sugar units and identifies them. Nine saccharides were detected.

## Overall composition

| Measure | Method | Result |
|---|---|---|
| Fraction at 1–2 million Daltons | SEC | ≥43% |
| Total polysaccharide content | SEC | 67% |
| Distinct saccharides detected | Glycosyl analysis | 9 |

The remaining ~57% sits below 1 million Daltons. Manapol® powder is a broad-spectrum, polydispersed aloe product: large polysaccharides can act as a prebiotic substrate in the intestine; smaller fractions are absorbed higher up.*

## Monosaccharide profile (glycosyl analysis)

| Saccharide | Note | Proportion |
|---|---|---|
| Mannose | Backbone of the acemannan polymer | 64.7% |
| Galacturonic acid | Indicates an aloe pectin structure | 13.8% |
| Glucose | | 7.1% |
| Galactose | | 4.9% |
| Xylose | | 3.9% |
| Rhamnose | | 1.7% |
| Arabinose | | 1.6% |
| Glucuronic acid | | 1.2% |
| Fucose | | 1.1% |

## Definition cited

The Chemical Abstracts Service (a division of the American Chemical Society) and the United States Adopted Names Council define acemannan by molecular weight at 1 to 2 million Daltons.

## Documents

- Product Information Sheet: https://library.mannatech.com/download/7158
- Supplement Facts: https://library.mannatech.com/download/8927
- Frequently Asked Questions: https://library.mannatech.com/download/12686

## Related

- Ambrotose® homepage: https://aloe.mannatech.com/llms.txt
- Manapol® powder: https://aloe.mannatech.com/manapol/llms.txt
- Ambrotose Complex vs LIFE: https://aloe.mannatech.com/ambrotose/llms.txt

*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.